MEM Alpha Modification is an alteration of MEM (Minimum Essential Medium). MEM was originally developed by Harry Eagle and from there modified in several ways (including the alpha modification) to suit many cell culture applications. It is used for cultivation of numerous suspension and adherent cells, including HeLa, BHK-21, HEK 293, HEP-2, HT-1080, MCF-7, fibroblasts, and primary rat astrocytes. This Alpha MEM modification comes without L-Glutamine and without Nucleosides. Liquid media provide basic nutrients for all cell culture applications. Each of our high-quality cell culture media is manufactured according to the originally published formula or modifications essential to the consistent performance and stability of the culture medium.