or the identification, cultivation and enumeration of yeast and molds in foodsApplications Identification / Cultivation / Enumeration YEAST & MOLDS Packaging Suspend 39 grams of medium in one liter of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Sterilize in autoclave at 118-121ºC for 15 minutes. Cool to 45-50ºC, mix well and dispense into plates. The prepared medium should be stored at 8-15°C. The color is amber, slightly opalescent.