FreezeMe One is a cryoprotective medium for cell culture that contains 10% DMSO (dimethyl sulfoxide) and 20% FBS. As a complete freezing medium, FreezeMe One can be used for, e.g. long-term storage of an established cell line or cell stock maintenance. DMSO and FBS serve as cryoprotective agents that reduce the freezing point of the medium. This allows a more sustainable cooling rate, reducing the potential damage done by ice crystal formation. The serum used is extensively tested to protect cells during cell preservation. FreezeMe One is a ready-to-use solution which is suitable for a broad spectrum of both adherent and suspension cells.