Ham's F-12 Medium (Ham's F-12 Nutrient Mix) is a widely used basal medium for cell culture. It was developed for serum-free, single-cell plating of Chinese Hamster Ovary (CHO) cells, lung cells, and mouse L cells. Ham’s F-12 is the medium of choice for a Clonal Toxicity Assay (CTA). This formulation of Ham's F-12 medium is supplemented with L-Glutamine. Liquid media provide basic nutrients for all cell culture applications. Each of our high-quality cell culture media is manufactured according to the originally published formula or modifications essential to the consistent performance and stability of the culture medium.