CHO|ONE A is a cultivation or adaptation medium for recombinant CHO cells in small-scale shaker bottles or spinner flasks. Lacking certain metabolites, CHO|ONE A exerts a strong selection pressure on the cells. This special formulation of CHO media simplifies and shortens the time-consuming adaptation to fed-batch culture. CHO|ONE A comes without Insulin & without L-Glutamine and is supplemented with Pluronic™. CHO|ONE A is part of the CHO|ONE Media System™, which contains all media and supplements necessary for the growth of CHO cells in batch or fed-batch processes. The CHO|ONE Media System™ was invented for small- and large-scale protein production. The CHO|ONE Media System™ was specially designed in close cooperation with our biopharmaceutical cooperation partner and is highly suitable for fed-batch cultures. It improves the yield and concentration of recombinant proteins through the optimization of a selection of essential nutrients and growth-promoting factors.