2×Hieff™ PCR Master Mix contains Hieff™ Taq DNA Polymerase (Cat#10101), dNTPs, and other PCR-required components. The Master Mix is stable for 3 months at 4°C with our customized stabilizers. The pre-mix solution is optimized for conventional PCR and ready to use by adding DNA template and primers. The PCR products can be loaded directly for electrophoresis with pre-loaded bromophenol blue dye. The amplified products contain 3 '-dA protrusion and can be easily cloned into T vector. The 2×HieffTM PCR Master Mix simplifies PCR procedure and reduces contamination.
Features
- Convenient, ready-to-use mix
- Thermostable—half-life is more than 40 min at 95°C
- Generates PCR products with 3'-dA overhangs
Applications
- Routine PCR amplification of DNA fragments
- High throughput PCR
- DNA labeling
Specifications
| Fidelity (vs. Taq) | 1 × |
| Hot Start | No |
| Overhang | 3'-A |
| Polymerase | Taq DNA Polymerase |
| Reaction Format | SuperMix or Master Mix |
| Reaction Speed | Standard |
| Product Type | PCR Master Mix (2×) |
Components
| Components No. | Name | 10102ES03 | 10102ES08 | 10102ES50 | 10102ES60 |
| 10102 | 2×Hieff™PCR Master Mix (With Dye) | 1 mL | 5×1 mL | 50×1 mL | 100×1 mL |
Shipping and Storage
The 2×Hieff™ PCR Master Mix products should be stored at -15℃ ~ -25℃ for 2 years.
Figures
Figure1. The expected 1.2 kb PCR products can be amplified with 2×HieffTM PCR Master Mix.
The Master Mix was stored at -20°C for 1 year following another 3 months at 4°C and 1 month at 25°C. Template: Arabidopsis genome. Annealing temperature: 60°C. Extension time: 40 sec.
Cited from "Ultrasensitive sensors reveal the spatiotemporal landscape of lactate metabolism in physiology and disease"
Cell Metab . 2023 Jan 3;35(1):200-211.e9. doi: 10.1016/j.cmet.2022.10.002
Manuals
10102ES08.pdf