MEM (Minimum Essential Medium) with Earle's Salts was originally developed by Harry Eagle and from there modified in several ways (e.g. alpha modification) to suit many cell culture applications. It is used for cultivation of numerous suspension and adherent cells, including HeLa, BHK-21, HEK 293, HEP-2, HT-1080, MCF-7, fibroblasts, and primary rat astrocytes. This modification of MEM powder with Earle's Salts is supplemented with L-Glutamine and comes without Sodium Bicarbonate (NaHCO3). Powder media can be reconstituted to provide basic nutrients for all cell culture applications. We offer dry powdered cell culture media and buffers which are manufactured using the highest quality ingredients. The powder is precisely blended in clean, controlled state-of-the-art equipment to eliminate contaminating dust and to control temperature and humidity.